Somatic embryogenesis and plant regeneration capacity in Argentinean maize (Zea mays L.) inbred lines
نویسندگان
چکیده
منابع مشابه
Genetic Diversity in Maize (Zea mays L.) Inbred Lines
Thirty yellow inbred lines of normal maize were evaluated for thirteen parameters at the experimental field of Hajee Mohammad Danesh Science and Technology University during 2010-11 to study the genetic divergence using multivariate analysis. The thirty inbreds fell into six distinct clusters. The intra-cluster distances in all the six clusters were more or less low, indicating that the genotyp...
متن کاملCallus Induction and Somatic Embryogenesis from Maize Mature Embryos (zea Mays L.)
Ten maize lines prevalently used by breeders in the maize program were screened for their ability to form type II callus. Mature seeds were used as explant for in vitro culturing. Inbred lines showed high embryogenic response and formed friable, type II calli. And also exhibited successful plant regeneration. Various culture media compositions based on Murashige and Skoog (1962) salts thus inve...
متن کاملMaize (Zea mays L.).
Agrobacterium tumefaciens-mediated transformation is an effective method for introducing genes into maize. In this chapter, we describe a detailed protocol for genetic transformation of the maize genotype Hi II. Our starting plant material is immature embryos cocultivated with an Agrobacterium strain carrying a standard binary vector. In addition to step-by-step laboratory transformation proced...
متن کاملGenome-Wide Association Study Identifies Candidate Genes That Affect Plant Height in Chinese Elite Maize (Zea mays L.) Inbred Lines
BACKGROUND The harvest index for many crops can be improved through introduction of dwarf stature to increase lodging resistance, combined with early maturity. The inbred line Shen5003 has been widely used in maize breeding in China as a key donor line for the dwarf trait. Also, one major quantitative trait locus (QTL) controlling plant height has been identified in bin 5.05-5.06, across severa...
متن کاملSomatic embryogenesis and plant regeneration in Gloriosa L.
Friable callus was initiated from shoot apices of Gloriosa superba L. on basal MS medium supplemented with 2, 4-D (4mg L(-1)) + Kn(5 mg L(-1)) + CH(10 mg L(-1)) + CW(20%). Subculture of callus on the same medium after 4-5 weeks showed induction of large number of somatic embryos, which was confirmed with histological studies. Development of embryoids in plantlet took place when the embryogenic ...
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ژورنال
عنوان ژورنال: Electronic Journal of Biotechnology
سال: 2012
ISSN: 0717-3458,0717-3458
DOI: 10.2225/vol15-issue1-fulltext-7